The research team of West China School of Stomatology has tested hundreds of Chinese herbs and identified that Galla Chinensis has a strong potential to prevent dental caries due to its antibacterial capacity and tooth mineralization benefit. Galla Chinensis also possesses substantial antiviral, anticancer, hepatoprotective, antidiarrheal and antioxidant activities. However, the main active ingredient of Galla Chinensis is unknown, which restricts the application in dentistry.

In the present study, several Galla Chinensis extracts with different main ingredients were obtained and determined by liquid chromatography-mass spectrometry (LC-MS) analysis. The antibacterial capacity was determined using the polymicrobial biofilms model, which can generate reproducible plaque-like biofilms that occur in vivo. The effect of inhibiting tooth demineralization was tested using an in vitro pH-cycling regime, which mimicked the periodic pH change in mouth.